Thirty-one patients undergoing matrix-associated autologous chondrocyte implantation (MACI) of the femoral condyle were randomized postoperatively to either accelerated or delayed weightbearing in their rehabilitation. The primary results were radiological and clinical outcome measures, using MRI and the International Knee Documentation Committee score (IKDC), the Tegner activity score, and the Knee Injury and Osteoarthritis Outcome Score (KOOS). The accelerated weightbearing protocol provided similar clinical and functional results to the delayed group without negatively affecting graft healing after 2 years follow-up.